|Unit Size||120 ml|
|Country of Manufacture||United States|
|Applications||Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot|
|Substrate Reaction Product||Precipitating Reaction Product|
|Microscopy||Brightfield, Spectral Imaging|
|Additional Substrate Properties||Contrast with Tissue Pigments, Multiple Labeling|
|Mounting||Aqueous (Hardening), Aqueous (Non-Hardening)|
AEC (3-amino-9-ethylcarbazole) and ImmPACT™ AEC HRP Substrates both produce red reaction products that can be used as a single label or as a second color for multiple antigen labeling. AEC and ImmPACT™ AEC chromogens can be used for both manual and automated staining methods. Stained slides must be aqueously mounted because these reaction products are soluble in organic solvents. Sections stained with AEC are stable for at least 4 years when mounted in VectaMount™ AQ (H-5501).
This product utilizes Vector Laboratories novel ImmPACT™ technology to generate a crisper, brighter red reaction product that is 3-5 times more sensitive than conventional AEC substrates such as AEC Substrate Kit (SK-4200). This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT™ AEC reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 47° C.