VECTOR NovaRED Peroxidase (HRP) Substrate Kit

Cat. No: SK-4800
Price: $150.00
Unit Size: 1 kit

Overview

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 300 ml of working solution
Cat. No. SK-4800
Unit Size 1 kit
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Red
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Multiple Labeling
Mounting Non-Aqueous (Permanent)

More Information

Vector® NovaRED™ and ImmPACT™ NovaRED™ HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted. Both chromogens are useful as alternatives to DAB or as a second color for multiple antigen labeling. Vector® NovaRED™ and ImmPACT™ NovaRED™ substrates also provide excellent color contrast in pigmented tissue such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield microscopy. Vector® NovaRED™ and ImmPACT™ NovaRED™ substrates can be used for both manual and automated staining methods.

Vector® NovaRED™ Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® NovaRED™ substrate is equivalent to Vector® DAB Substrate, and 4 times greater than AEC. This product can also be used on blots. This kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 °C, this kit is stable for one year.

Relative Sensitivity of Substrates

Substrate Combinations

 

Counterstain Compatibility