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Vector NovaRED® Substrate Kit, Peroxidase (HRP) (SK-4800)

Tonsil: CD20 (m), ImmPRESS HRP Anti-Mouse IgG, Vector NovaRED Substrate.
Mouse, Newborn (eye, double label): GFAP (m), MOM Peroxidase Kit, Vector NovaRED substrate (red); Synapsin (m), MOM Peroxidase Kit, DAB+Ni substrate (gray/black). Note contrast with endogenous pigment (brown) seen in surrounding tissues.
Mouse, Newborn (tongue, double label): Synapsin (m), MOM Peroxidase kit, Vector NovaRED substrate (red); Desmin (m), MOM Peroxidase Kit, DAB+Ni substrate (gray/black).
Vector NovaRED® Substrate Kit, Peroxidase (HRP)
Vector NovaRED® Substrate Kit, Peroxidase (HRP)
Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP kit, Vector Blue substrate (blue).
Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red).
Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).
Tonsil (double label): CD3 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red).
Lymphoma: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40; Cat. No. VP-M671), ImmPress anti-mouse Ig reagent (MP-7402), and Vector NovaRED peroxidase substrate (SK-4800; red). Hematoxylin QS counterstain (blu
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40; Cat. No. VP-M671), ImmPress anti-mouse Ig reagent (MP-7402), and Vector NovaRED peroxidase substrate (SK-4800; red). Hematoxylin QS countersta
Peyers Patch: ImmPRESS Anti-Rabbit Ig using Ki67 rabbit primary antibody with Vector NovaRed (red) substrate. Hematoxylin QS counterstain (blue).
Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown).
Breast Cancer (double Label): Epithelium (red, Vector NovaRED), endothelium (blue, Vector Blue).
Tonsil: Ki67 antigen detected using ImmPRESS Universal Reagent and Vector NovaRED (red) substrate. Hematoxylin QS (blue) counterstain.
Tonsil: CD3 antigen detected using ImmPRESS Universal Reagent and Vector NovaRED (red) substrate. Hematoxylin QS (blue) counterstain.
Small Intestine (double label): ImmPRESS Anti-Mouse Ig using CD10 primary antibody with DAB/Ni (black) substrate, and ImmPRESS Anti-Mouse Ig using Cytokeratin 20 primary antibody with Vector NovaRED (red) substrate. No counterstain.
Breast Cancer (double label): Endothelium (red, Vector NovaRED), epithelium (blue, Vector Blue).
Breast Cancer (triple label): Estrogen receptor (red, Vector NovaRED), endothelium (brown, Vector DAB), epithelium (blue/gray, Vector SG).
Colon cancer: COX-2 rabbit monoclonal antibody detected with ImmPRESS Universal Reagent and Vector NovaRED substrate (red). Hematoxylin QS counterstain (blue). Formalin-fixed, paraffin embedded tissue section.
Tonsil: AE1/AE3 (m), ImmPRESS HRP Anti-Mouse IgG, Vector NovaRED Substrate.
Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector NovaRED substrate (red); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector DAB+Ni substrate (gray/black).
Small Bowel (double label): Peripherin (m), ImmPRESS Anti-Mouse Ig Kit, Vector NovaRED (red) substrate. Desmin (m), ImmPRESS Anti-Mouse Ig Kit, DAB/Ni (black) substrate.
Colon (double label): M2A Antigen (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).
Vector NovaRED® Substrate Kit, Peroxidase (HRP)
Tonsil: CD20 (m), ImmPRESS HRP Anti-Mouse IgG, Vector NovaRED Substrate.
Mouse, Newborn (eye, double label): GFAP (m), MOM Peroxidase Kit, Vector NovaRED substrate (red); Synapsin (m), MOM Peroxidase Kit, DAB+Ni substrate (gray/black). Note contrast with endogenous pigment (brown) seen in surrounding tissues.
Mouse, Newborn (tongue, double label): Synapsin (m), MOM Peroxidase kit, Vector NovaRED substrate (red); Desmin (m), MOM Peroxidase Kit, DAB+Ni substrate (gray/black).
Vector NovaRED® Substrate Kit, Peroxidase (HRP)
Vector NovaRED® Substrate Kit, Peroxidase (HRP)
Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP kit, Vector Blue substrate (blue).
Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red).
Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).
Tonsil (double label): CD3 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red).
Lymphoma: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40; Cat. No. VP-M671), ImmPress anti-mouse Ig reagent (MP-7402), and Vector NovaRED peroxidase substrate (SK-4800; red). Hematoxylin QS counterstain (blu
Colon submucosa: Lymphatic endothelium stained using mouse monoclonal antibody against M2A antigen (clone D2-40; Cat. No. VP-M671), ImmPress anti-mouse Ig reagent (MP-7402), and Vector NovaRED peroxidase substrate (SK-4800; red). Hematoxylin QS countersta
Peyers Patch: ImmPRESS Anti-Rabbit Ig using Ki67 rabbit primary antibody with Vector NovaRed (red) substrate. Hematoxylin QS counterstain (blue).
Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown).
Breast Cancer (double Label): Epithelium (red, Vector NovaRED), endothelium (blue, Vector Blue).
Tonsil: Ki67 antigen detected using ImmPRESS Universal Reagent and Vector NovaRED (red) substrate. Hematoxylin QS (blue) counterstain.
Tonsil: CD3 antigen detected using ImmPRESS Universal Reagent and Vector NovaRED (red) substrate. Hematoxylin QS (blue) counterstain.
Small Intestine (double label): ImmPRESS Anti-Mouse Ig using CD10 primary antibody with DAB/Ni (black) substrate, and ImmPRESS Anti-Mouse Ig using Cytokeratin 20 primary antibody with Vector NovaRED (red) substrate. No counterstain.
Breast Cancer (double label): Endothelium (red, Vector NovaRED), epithelium (blue, Vector Blue).
Breast Cancer (triple label): Estrogen receptor (red, Vector NovaRED), endothelium (brown, Vector DAB), epithelium (blue/gray, Vector SG).
Colon cancer: COX-2 rabbit monoclonal antibody detected with ImmPRESS Universal Reagent and Vector NovaRED substrate (red). Hematoxylin QS counterstain (blue). Formalin-fixed, paraffin embedded tissue section.
Tonsil: AE1/AE3 (m), ImmPRESS HRP Anti-Mouse IgG, Vector NovaRED Substrate.
Tonsil (double label): CD3 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector NovaRED substrate (red); Ki67 (m), ImmPRESS Anti-Mouse Ig Reagent, Vector DAB+Ni substrate (gray/black).
Small Bowel (double label): Peripherin (m), ImmPRESS Anti-Mouse Ig Kit, Vector NovaRED (red) substrate. Desmin (m), ImmPRESS Anti-Mouse Ig Kit, DAB/Ni (black) substrate.
Colon (double label): M2A Antigen (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).
Vector NovaRED® Substrate Kit, Peroxidase (HRP)
[text-backorder]

Description

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 300 ml of working solution

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorRed
MicroscopyBrightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Multiple Labeling
MountingNon-Aqueous (Permanent)

Kit Contents:

  • 6 ml Vector NovaRED Reagent 1
  • 3.5 ml Vector NovaRED Reagent 2
  • 3.5 ml Vector NovaRED Reagent 3
  • 5 ml Vector NovaRED Reagent 4

Documents

Citations

Technical Information

Vector NovaRED and ImmPACT® NovaRED HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted. Both chromogens are useful as alternatives to DAB or as a second color for multiple antigen labeling. Vector NovaRED and ImmPACT NovaRED substrates also provide excellent color contrast in pigmented tissue such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield microscopy. Vector NovaRED and ImmPACT NovaRED substrates can be used for both manual and automated staining methods.

Vector NovaRED Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector NovaRED substrate is equivalent to Vector DAB Substrate, and 4 times greater than AEC. This product can also be used on blots. This kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 °C, this kit is stable for one year.

SUBSTRATEsensitivity2 jpg

Substrate.CounterstainChart jpg SubstrateCombos jpg

Applicable patents and legal notices are available at legal notices.

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