VECTOR Blue Alkaline Phosphatase (Blue AP) Substrate Kit

Cat. No: SK-5300
Price: $155.00
Unit Size: 1 kit

Overview

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • One year expiry date
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation:  broad, peak at ~500 nm*
  • Emission:  broad, peak at ~680 nm*

*Histochem J. (1998) 8:577

Cat. No. SK-5300
Unit Size 1 kit
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Color of Fluorescence Far Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

More Information

The Vector® Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. With the aid of imaging systems and software, the spectral profile of this substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Vector® Blue is also fluorescent. Sections stained with the Vector® Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount , H-5000) must be used because the reaction product is soluble in xylene. Vector® Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.

Relative Sensitivity of Substrates

Substrate Combinations

 

Counterstain Compatibility

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