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ImmPACT SG Peroxidase (HRP) Substrate

Catalog Number: SK-4705

Availability: In stock

$135.00
Unit Size: 120 ml

Tonisl:  Cytokeratin AE1/AE3 (m), ImmPRESS Reagent (HRP), ImmPACT SG (blue-gray). Zoom

Overview

    Features:

    • 2-4 fold greater sensitivity than original substrate kit
    • Optimized for IHC/ICC/ISH and blots
    • Permanent mounting
    • Ideal for single and multiple labeling
    • Working solutions stable for up to 14 days at 4 °C
    • One year expiry date
    • Supplied as 120 ml of diluent and stock solutions in dropper bottles

    Additional Information

    Catalog Number SK-4705
    Unit Size 120 ml
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
    Detection Enzyme(s) Peroxidase
    Substrate Reaction Product Precipitating Reaction Product
    Chromogen Color Blue-Gray
    Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
    Additional Substrate Properties Contrast with Tissue Pigments, Multiple Labeling
    Mounting Aqueous (Hardening), Non-Aqueous (Permanent)
Additional Info

    Details

    Vector® SG and ImmPACT™ SG HRP substrates produce blue-gray reaction products that can be used as a single label or as a second color for multiple antigen labeling. With the aid of imaging systems and software, the spectral profile of both chromogens can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield or electron microscopy. Vector® SG and ImmPACT™ SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted.

    This product utilizes Vector Laboratories novel ImmPACT™ technology to generate an intense blue-gray reaction product that is 2-3 times more sensitive than Vector® SG substrate kit (SK-4700). This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT™ SG reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 °C.

    Relative Sensitivity of Substrates

    Substrate Combinations

     

    Counterstain Compatibility

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