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BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium

Catalog Number: SK-5400

Availability: In stock

$135.00
Unit Size: 1 kit

Prostate:  PSA (m), VECTASTAIN ABC-AP Mouse IgG Kit, BCIP/NBT (indigo), viewed under bright field microscopy. Zoom

Overview

    Features:

    • Greater sensitivity than conventional substrates
    • Consistent and reliable
    • Ideal for IHC/ICC/ISH and blots
    • Fluorescent
    • Heat Stable
    • Permanent mounting
    • Ideal for single and multiple labeling
    • One year expiry date
    • Stock solutions supplied in convenient dropper bottles promoting ease of handling
    • No wait times for mixing and dissolving powders or tablets
    • Sufficient reagents to produce 200 ml of working solution
    • Excitation:  645-685 nm*
    • Emission:  peaks at 823 and 855 nm*

     *Biotechniques (2007) 42:756-759 and BioTechniques (2014) 57:254-256

    Additional Information

    Catalog Number SK-5400
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
    Detection Enzyme(s) Alkaline Phosphatase
    Substrate Reaction Product Precipitating Reaction Product
    Chromogen Color Indigo
    Color of Fluorescence Far Red
    Microscopy Brightfield, Fluorescence, Spectral Imaging
    Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
    Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Additional Info

    Details

    BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product that can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. This substrate can also be used for developing blots or for in situ hybridization. At 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. This kit contains stock solutions in convenient dropper bottles.

    Relative Sensitivity of Substrates

    Substrate Combinations

     

    Counterstain Compatibility

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