ImmPACT Vector Red Alkaline Phosphatase (AP) Substrate

Cat. No: SK-5105
Price: $134.00
Unit Size: 120 ml

Overview

Features:

  • The most sensitive red AP substrate
  • 2-4 fold greater sensitivity than original substrate kit
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • One year expiry date
  • Supplied as 120 ml of ready-to-use diluent and stock solutions in dropper bottles
  • Excitation:  broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission:  Above 560 nm
  • Color:  Red
Cat. No. SK-5105
Unit Size 120 ml
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Magenta
Color of Fluorescence Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

More Information

ImmPACT™ Vector® Red AP Substrate Kit, a new formulation for the Vector® Red AP substrate, produces a bright red reaction product that is crisper and is 2-4 times more sensitive than the original Vector® Red substrate kit. This product consists of 120 ml of ready-to-use diluent and concentrated stock solutions in convenient dropper bottles.

Both Vector® Red and ImmPACT™ Vector® Red alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. Preparations stained with either Vector® Red or ImmPACT™ Vector® Red AP chromogen can be dehydrated, cleared, and permanently mounted. Both chromogen formulations can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of Vector® Red or ImmPACT™ Vector® Red substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Both formulations can be used for either manual or automated staining methods.

The bright fluorescence of the Vector® Red or ImmPACT™ Vector® Red reaction products are visible using DyLight® 594, Texas Red® or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes.

Either substrate formulation can also be used on blots. Compared to other AP substrates, the Vector® Red and ImmPACT™ Vector® Red reaction products have a wider dynamic range, offering better quantitation of targets on blots.

Relative Sensitivity of Substrates

Substrate Combinations

 

Counterstain Compatibility