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ImmPACT VIP Peroxidase (HRP) Substrate

Catalog Number: SK-4605

Availability: In stock

$135.00
Unit Size: 120 ml

Tonsil:  CD20 (m), ImmPRESS Anti-Mouse Ig Kit, ImmPACT VIP (purple) substrate.  Zoom

Overview

    Features:

    • 2-4 fold greater sensitivity than original substrate kit
    • Optimized for IHC/ICC/ISH and blots
    • Permanent mounting
    • Ideal for single and multiple labeling
    • Working solutions stable for up to 14 days at 4 °C
    • One year expiry date
    • Supplied as 120 ml of diluent and stock solutions in dropper bottles

    Product review on Biocompare.com.

    1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

    Additional Information

    Catalog Number SK-4605
    Unit Size 120 ml
    CE Marked Yes
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
    Detection Enzyme(s) Peroxidase
    Substrate Reaction Product Precipitating Reaction Product
    Chromogen Color Purple
    Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
    Additional Substrate Properties Contrast with Tissue Pigments, Multiple Labeling
    Mounting Non-Aqueous (Permanent)
Additional Info

    Details

    Vector® VIP and ImmPACT™ VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector® VIP and ImmPACT™ VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector® VIP and ImmPACT™ VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

    This product utilizes Vector Laboratories novel ImmPACT™ technology to generate an intense violet reaction product that is 2-4 times more sensitive than Vector ®VIP substrate kit (SK-4600). This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT™ VIP reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 °C.

    Product review on Biocompare.com.

    1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

    Relative Sensitivity of Substrates

    Substrate Combinations

     

    Counterstain Compatibility

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