ImmPACT VIP Peroxidase (HRP) Substrate

Cat. No: SK-4605
Price: $144.00
Unit Size: 120 ml

Overview

Features:

  • 2-4 fold greater sensitivity than original substrate kit
  • Optimized for IHC/ICC/ISH and blots
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Working solutions stable for up to 14 days at 4 °C
  • One year expiry date
  • Supplied as 120 ml of diluent and stock solutions in dropper bottles

Product review on Biocompare.com.

1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

Cat. No. SK-4605
Unit Size 120 ml
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Purple
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Multiple Labeling
Mounting Non-Aqueous (Permanent)

More Information

Vector® VIP and ImmPACT™ VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector® VIP and ImmPACT™ VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector® VIP and ImmPACT™ VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

This product utilizes Vector Laboratories novel ImmPACT™ technology to generate an intense violet reaction product that is 2-4 times more sensitive than Vector ®VIP substrate kit (SK-4600). This kit consists of 120 ml of diluent and concentrated stock solutions of ImmPACT™ VIP reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 °C.

Product review on Biocompare.com.

1.  Duncan SM, Seigel GM. High-contrast enzymatic immunohistochemistry of pigmented tissues. J Biol Methods2016;3(3):e47. doi: 10.14440/jbm.2016.122

Relative Sensitivity of Substrates

Substrate Combinations

 

Counterstain Compatibility