DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine)

SK-4100
SKU Unit Size Price Qty
SK-4100 1 kit
$163.00

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Description

The DAB substrate reacts with HRP to yield an insoluble brown-colored product. This kit includes nickel, providing the option of changing the reaction product from brown to gray-black.

Features:

  • Ideal for IHC/ICC/ISH and blots
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Brown, Gray-Black
Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate Properties Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Non-Aqueous (Permanent)
Safety Title WARNING: Cancer - www.P65Warnings.ca.gov

Kit Components

Kit Contents:

  • 6 ml DAB Reagent 1
  • 6 ml DAB Reagent 2
  • 6 ml DAB Reagent 3
  • 6 ml DAB Reagent 4 (Nickel Optional)

Documents

Product FAQs

I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?

Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

Citations

Technical Information

This kit contains stock solutions in convenient dropper bottles. Slides developed with DAB/ Ni should be dehydrated, cleared, and permanently mounted. 

Product review on Biocompare.com.



 

 

 

 

Home Horseradish Peroxidase (HRP) Substrates DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine)