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DAB Peroxidase (HRP) Substrate Kit (with Nickel), 3,3’-diaminobenzidine

Catalog Number: SK-4100

Availability: In stock

Unit Size: 1 kit

Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate. Zoom



    • Greater sensitivity than conventional substrates
    • Consistent and reliable
    • Ideal for IHC/ICC/ISH and blots
    • Heat Stable
    • Permanent mounting
    • Ideal for single and multiple labeling
    • Stock solutions supplied in convenient dropper bottles promoting ease of handling
    • No wait times for mixing and dissolving powders or tablets
    • Includes nickel to produce a gray-black rather than brown reaction product
    • One year expiry date
    • Sufficient reagents to produce 300 ml of working solution

    Additional Information

    Catalog Number SK-4100
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
    Detection Enzyme(s) Peroxidase
    Substrate Reaction Product Precipitating Reaction Product
    Chromogen Color Brown, Gray-Black
    Microscopy Brightfield, Darkfield, Electron, Spectral Imaging
    Additional Substrate Properties Heat Resistant, Multiple Labeling
    Mounting Aqueous (Hardening), Non-Aqueous (Permanent)
Additional Info


    DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).

    This kit contains stock solutions in convenient dropper bottles. This kit includes nickel, providing the option of changing the reaction product from brown to gray-black and increasing sensitivity in blot applications. Slides developed with DAB/ Ni should be dehydrated, cleared, and permanently mounted. This product can also be used on blots. This kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4°C, this kit is stable for one year.

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