VECTOR Red Alkaline Phosphatase (Red AP) Substrate Kit

Cat. No: SK-5100
Price: $155.00
Unit Size: 1 kit

Overview

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent Mounting
  • Ideal for single and multiple labeling
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • One year expiry date
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation:  broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission:  Above 560 nm
  • Color:  Red
Cat. No. SK-5100
Unit Size 1 kit
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Magenta
Color of Fluorescence Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

More Information

Vector® Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector® Red substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of its stability under ISH conditions, Vector® Red can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of this substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized.

The bright red to orange fluorescence of the Vector Red reaction product can be visualized using DyLIght® 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes. This substrate can also be used for in situ hybridization or on blots, where it has a wider dynamic range than other substrates.

This kit contains stock solutions in convenient dropper bottles.

Relative Sensitivity of Substrates

Substrate Combinations

 

Counterstain Compatibility