Immunohistochemistry Resource Guide

Not enough time read now ?

Click Here to Download This Entire Guide
Mouse on Mouse (M.O.M.™) Immunodetection Kits

Vector Laboratories M.O.M.™ Immunodetection systems are specifically designed to localize mouse primary antibodies on mouse tissue while avoiding background staining. These M.O.M.™ Kits contain our proprietary M.O.M.™ Mouse Ig Blocking Reagent. M.O.M.™ Kits are available based on either avidin-biotin technology (M.O.M.™ Elite® ABC Kit, Fluorescein Kit, or Basic Kit) or polymer technology (M.O.M.™ ImmPRESS™ HRP Polymer Kit). Use the M.O.M.™ Immunodetection systems to introduce two or more different labels using a multiple antigen labeling protocol. You can detect several mouse primary antibodies on the same tissue section, regardless of the species of the tissue. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M.™ System.

  • Significantly reduces endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  • Simple protocols
  • Eliminates tedious calculations
  • Eliminates primary antibody prebinding steps
  • Clear, crisp, specific staining of antigens of interest
  • Compatible with fluorescent or enzyme-based detection
  • Available with or without enzyme or fluorochrome
Recommended applications:
chevron-right Studies in genetically engineered mice
chevron-right Transgenic and knock-out models
chevron-right Mouse xenograft tissue
chevron-right Normal mouse tissue

Sections of mouse kidney stained with mouse antibody against smooth muscle actin using VECTASTAIN® ABC-AP Kit and Vector® Blue substrate. Using standard biotinylated anti-mouse antibody and normal blocking serum, confusing background is seen (left). With the Vector® M.O.M.™ Basic Kit, clean background and specific staining is achieved (right).

Mouse intestine stained with standard anti-mouse IgG polymer system (left) and Vector® M.O.M.™ ImmPRESS™ HRP Polymer Kit (right). Brown signal indicates IgG background staining. (Both: No primary antibody, Vector® DAB stain, hematoxylin counterstain.)

ProductCatalog Number
M.O.M.™ Peroxidase Kit PK-2200
M.O.M.™ Fluorescein Kit FMK-2201
M.O.M.™ Basic Kit BMK-2202
M.O.M.™ ImmPRESS™ HRP Polymer Kit MP-2400
M.O.M.™ Mouse Ig Blocking Reagent MKB-2213
M.O.M.™ Biotinylated Anti-Mouse Ig Reagent* MKB-2225
M.O.M.™ ImmPRESS™ HRP Polymer Anti-Mouse Reagent MPX-2402

* This reagent must be used with the M.O.M.™ Mouse Ig Blocking Reagent (MKB-2213). It is not intended to be a stand-alone reagent for mouse on mouse applications.

17