Table of Contents
- Introduction
- Immunohistochemistry Workflow
- Immunohistochemistry Selection Guide
- Pioneering in IHC Technology
- Choosing a Detection System
- Avidin-Biotin Complex (ABC)-Based Detection
- VECTASTAIN® ABC Systems
- VECTASTAIN® ABC Kits
- Choosing a VECTASTAIN® ABC Kit
- > Customizing your VECTASTAIN® ABC Kit
- Polymer-Based Detection
- ImmPRESS® One-Step Polymer Systems
- > ImmPRESS® Two-Step Amplified Polymer Systems
- > Choosing an ImmPRESS® Polymer Kit
- > Multiple Antigen Labeling Simplified
- Species on Species Detection
- M.O.M.® (Mouse on Mouse) Immunodetection Kits
- Choosing an Enzyme Substrate
- Enzyme Substrates
- > Enzyme Substrate Properties
- Multiple Antigen Labeling
- Enzyme Substrate Combinations
- Counterstaining
- > Counterstain/Substrate Compatibility Table
- Blocking Background Signal
- Secondary and Tertiary Detection Reagents
- Mounting Media
- Accessory Reagents
- Introduction
- Immunohistochemistry Workflow
- Immunohistochemistry Selection Guide
- Pioneering in IHC Technology
- Choosing a Detection System
- Avidin-Biotin Complex (ABC)-Based Detection
- VECTASTAIN® ABC Systems
- VECTASTAIN® ABC Kits
- Choosing a VECTASTAIN® ABC Kit
- > Customizing your VECTASTAIN® ABC Kit
- Polymer-Based Detection
- ImmPRESS® One-Step Polymer Systems
- > ImmPRESS® Two-Step Amplified Polymer Systems
- > Choosing an ImmPRESS® Polymer Kit
- > Multiple Antigen Labeling Simplified
- Species on Species Detection
- M.O.M.® (Mouse on Mouse) Immunodetection Kits
- Choosing an Enzyme Substrate
- Enzyme Substrates
- > Enzyme Substrate Properties
- Multiple Antigen Labeling
- Enzyme Substrate Combinations
- Counterstaining
- > Counterstain/Substrate Compatibility Table
- Blocking Background Signal
- Secondary and Tertiary Detection Reagents
- Mounting Media
- Accessory Reagents
Multiple Antigen Labeling
Localization of two or more antigens on the same tissue section is a powerful research tool that can provide valuable insights into cellular biochemistry, protein-protein interactions, and spatial relationships of biomarkers. Our detection systems and enzyme substrates have been developed and rigorously tested to deliver the high sensitivity, low background, and extreme clarity that is required to differentiate multiple epitopes simultaneously. You can choose to use the same enzyme system with different substrates or different enzyme systems and their respective substrates. For a detailed description of these applications, protocols, and additional images please visit our website or request a free copy of our guide, Discovery Through Color: IHC Multiplexing Guide.
Cytokeratin (AE1/AE3, m), ImmPRESS®-AP Anti-Mouse IgG Reagent, Vector® Blue AP Substrate (blue) • CD3 (rb), ImmPRESS Anti-Rabbit IgG HRP Reagent, ImmPACT® AMEC Red HRP Substrate (red).