Immunohistochemistry Resource Guide
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Table of Contents
Immunohistochemistry Selection Guide
Avidin-Biotin Complex (ABC)-Based Detection
Choosing a VECTASTAIN® ABC Kit
> Customizing your VECTASTAIN® ABC Kit
ImmPRESS® One-Step Polymer Systems
> ImmPRESS® Two-Step Amplified Polymer Systems
> Choosing an ImmPRESS® Polymer Kit
> Multiple Antigen Labeling Simplified
M.O.M.® (Mouse on Mouse) Immunodetection Kits
> Counterstain/Substrate Compatibility Table
Counterstain/Substrate Compatibility Table
This table is designed as a reference to determine the optimal counterstain/substrate combination for your application. Considerations should be given to tissue type, antigen unmasking protocol and other detection parameters to achieve the desired staining intensity.
| Substrate | Catalog Number | Vector® Hematoxylin and Hematoxylin QS H-3401 and H-3404 | Vector® Methyl Green H-3402 | Vector® Nuclear Fast Red H-3403 |
|---|---|---|---|---|
| ImmPACT® DAB EqV | SK-4103 | Excellent Contrast | Excellent Contrast | Fair Contrast |
| ImmPACT® DAB (brown) | SK-4105 | Excellent Contrast | Excellent Contrast | Fair Contrast |
| Vector® DAB (brown) | SK-4100 | Excellent Contrast | Excellent Contrast | Fair Contrast |
| Vector® DAB-Ni (gray-black) | SK-4100 | Excellent Contrast | Fair Contrast * | Good Contrast |
| ImmPACT® AEC (red) | SK-4205 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
| ImmPACT® AMEC Red (red) | SK-4285 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
| Vector® AEC (red) | SK-4200 | Excellent Contrast | Counterstain Incompatibility ** | Color Incompatibility |
| TMB (blue) | SK-4400 | Color Incompatibility | Counterstain Incompatibility | Excellent Contrast |
| ImmPACT® VIP (purple) | SK-4605 | Fair Contrast | Excellent Contrast | Poor Contrast |
| Vector® VIP (purple) | SK-4600 | Fair Contrast | Excellent Contrast | Poor Contrast |
| ImmPACT® SG (blue-gray) | SK-4705 | Poor Contrast | Good Contrast | Excellent Contrast |
| Vector® SG (blue-gray) | SK-4700 | Poor Contrast | Good Contrast | Excellent Contrast |
| ImmPACT® NovaRED® (red) | SK-4805 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
| Vector® NovaRED® (red) | SK-4800 | Excellent Contrast | Excellent Contrast *** | Color Incompatibility |
| ImmPACT® Vector® Red (magenta) | SK-5105 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
| Vector® Red (magenta) | SK-5100 | Excellent Contrast | Excellent Contrast | Color Incompatibility |
| Vector® Black (black) | SK-5200 | Excellent Contrast | Excellent Contrast * | Excellent Contrast |
| Vector® Blue (blue) | SK-5300 | Color Incompatibility | Good Contrast | Excellent Contrast |
| BCIP/NBT (indigo) | SK-5400 | Color Incompatibility | Excellent Contrast * | Excellent Contrast |
* This substrate shows a slight decrease in sensitivity following the methyl green protocol. This decrease can be minimized by reducing the heat incubation and acetone rinse times in the methyl green protocol.
** Substrate dissolves in acetone wash.
*** A slight color change in ImmPACT NovaRED and Vector NovaRED reaction product may be seen using methyl green.
| Product | Mountants | Catalog Number |
|---|---|---|
| Vector® Hematoxylin | Non-aqueous and Aqueous | H-3401-500 |
| Vector® Hematoxylin QS | Non-aqueous and Aqueous | H-3404-100 |
| Vector® Methyl Green | Non-aqueous | H-3402-500 |
| Vector® Nuclear Fast Red | Non-aqueous and Aqueous | H-3403-500 |
