Immunohistochemistry Resource Guide

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Counterstaining

A counterstain introduces color to specific cellular structures to provide contrast to the colored enzyme substrate. Counterstaining aids in visualization and target localization, facilitating interpretation of morphology and cell structure within the tissue section. Our nuclear counterstains are packaged as convenient, ready-to-use solutions for use on individual slides or in staining dishes.

Vector® Hematoxylin (blue)

  • Based on Gill’s III formulation
  • Progressive stain formula. The intensity can be adjusted to optimize results for either manual or automated systems
  • Excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mounting media
  • Alcohol- and mercury-free

Tumor tissue section showing specific cytoplasmic cell staining (Vector® DAB, brown) with Vector® Hematoxylin QS counterstain (blue).

Vector® Hematoxylin QS (blue)

  • Modification of Mayer’s hematoxylin developed especially for immunocytochemistry
  • Ready-to-use without filtration or ‘blueing’ step
  • Stains in less than 45 seconds
  • Excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mounting media
  • Mercury-free

Tonsil section showing specific cell staining (Vector® VIP, purple) with Vector® Methyl Green counterstain (green).

Vector® Nuclear Fast Red (pink)

  • Fast one-step protocol
  • Excellent alternative in multiple antigen labeling when hematoxylin obscures the substrate colors
  • Good contrast with a variety of substrates

Tumor tissue section showing specific cytoplasmic cell staining (Vector® SG, blue/ gray) with Vector® Nuclear Fast Red counterstain (red).

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