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TMB Substrate Kit, Peroxidase (HRP), (3,3', 5,5'-tetramethylbenzidine)

SK-4400
SKU Unit Size Price Qty
SK-4400 1 kit
$185.00
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Description

TMB (3,3', 5,5' tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.

Features:

  • Greater sensitivity than conventional substrates
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Microscopy Brightfield, Spectral Imaging
Mounting Non-Aqueous (Permanent)
Safety Title WARNING: Cancer - www.P65Warnings.ca.gov

Kit Components

What's in the box:

  • 6 ml TMB Reagent 1
  • 6 ml TMB Reagent 2
  • 6 ml TMB Reagent 3
  • 6 ml TMB Reagent 4

Documents

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Technical Information

TMB (3,3', 5,5' tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

Relative sensitivity of substrates in Immunohistochemistry: Vector Laboratories

Counterstain / Substrate Compatibility

This table is designed as a reference to determine the optimal counterstain / substrate combination for your application. Consideration should be given to tissue type, antigen unmasking protocol, and other detection parameters to achieve the desired staining intensity.

Substrate Catalog Number Hematoxylin (H-3404)
Hematoxylin QS (H-3401)		 Methyl Green (H-3402) Nuclear Fast Red (H-3404)

ImmPACT DAB (brown)

 SK-4105 Excellent Contrast Excellent Contrast Fair Contrast

ImmPACT DAB EqV (brown)

 SK-4103 Excellent Contrast Excellent Contrast Fair Contrast

DAB (brown)

 SK-4100 Excellent Contrast Excellent Contrast Fair Contrast

DAB-Ni (gray-black)

 SK-4100 Excellent Contrast Fair Contrast* Good Contrast

ImmPACT AEC (red)

 SK-4205 Excellent Contrast Counterstain Incompatibility** Color Incompatibility

ImmPACT AMEC Red (red)

 SK-4285 Excellent Contrast Counterstain Incompatibility** Color Incompatibility

AEC (red)

 SK-4200 Excellent Contrast Counterstain Incompatibility** Color Incompatibility

TMB (blue)

 SK-4400 Color Incompatibility Counterstain Incompatibility Excellent Contrast

ImmPACT VIP (purple)

 SK-4605 Fair Contrast Excellent Contrast Poor Contrast

Vector VIP (purple)

 SK-4600 Fair Contrast Excellent Contrast Poor Contrast

ImmPACT SG (blue-gray)

 SK-4705 Poor Contrast Good Contrast Excellent Contrast

SG (blue-gray)

 SK-4700 Poor Contrast Good Contrast Excellent Contrast

ImmPACT NovaRED (red)

 SK-4805 Excellent Contrast Excellent Contrast*** Color Incompatibility

Vector NovaRED (red)

 SK-4800 Excellent Contrast Excellent Contrast*** Color Incompatibility

ImmPACT Vector Red (magenta)

 SK-5105 Excellent Contrast Excellent Contrast Color Incompatibility

Vector Red (magenta)

 SK-5100 Excellent Contrast Excellent Contrast Color Incompatibility

Vector Black (black)

 SK-5200 Excellent Contrast Excellent Contrast* Excellent Contrast

Vector Blue (blue)

 SK-5300 Color Incompatibility Good Contrast Excellent Contrast

BCIP / NBT (indigo)

 SK-5400 Color Incompatibility Excellent Contrast* Excellent Contrast

*This substrate shows a slight decrease in sensitivity following the methyl green protocol. This decrease can be minimized by reducing the heat incubation and acetone rinse times.
**Substrate dissolves in acetone wash.
***A slight color change in ImmPACT NovaRED and Vector NovaRED reaction produt may be seen using methyl green.
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