Fluorescein Avidin D, FITC (A-2001-5)

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Description

Avidin D, Fluorescein labeled, is made from our highly purified Avidin D by a special labeling technique. This product has an optimal fluorochrome to protein ratio and is the reagent we recommend for routine immunofluorescence applications. Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining. 

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size5 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution10mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Recommended Storage2-8 °C
Concentration5.0 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
FormatConcentrate

Citations

Technical Information

For even higher sensitivity and specific applications, see Fluorescein Avidin DCS.

Applicable patents and legal notices are available at legal notices.

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