Streptavidin is a 60 kDa non-glycosylated protein composed of four identical subunits, each of which has a binding site for biotin. Our streptavidin is isolated from Streptomyces avidinii by a specific affinity system which results in a homogeneous, ultrapure streptavidin with very low nonspecific binding characteristics.
Unconjugated streptavidin is recommended for use in solid-phase assays and immunohistochemical applications when the technique of sequential addition of reagents (sandwich technique) is employed. This reagent has also been effectively employed in subtractive hybridization procedures.
|Unit Size||1 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, ELISAs|
|Recommended Usage||Recommended method of reconstitution: dissolve in 10 mM phosphate, 0.15 M NaCl, pH 7.5. Reconstituted solutions of streptavidin may be preserved with 0.08% sodium azide. The recommended concentration range for use is 5-20 µg/ml.|
|Recommended Storage||2-8 °C|
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