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For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:
Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC| Unit Size | 1 mg |
|---|---|
| Applications | Blotting Applications, Elispot, ELISAs |
| Recommended Usage | Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications. |
| Solution | 0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative |
| Recommended Storage | 2-8 °C, DO NOT FREEZE |
| Concentration | 1.0 mg/ml active conjugate |
| Detection Enzyme(s) | Peroxidase |
| Conjugate | Peroxidase |
| Format | Concentrate |
Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.
This Horseradish peroxidase streptavidin preparation (HRP-Sav; SA-5014) is optimized for use in solid-phase assays such as ELISAs and blotting applications.
For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:
Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC
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