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Avidin D, Fluorescein labeled, is made from our highly purified Avidin D by a special labeling technique. This product has an optimal fluorochrome to protein ratio and is the reagent we recommend for routine immunofluorescence applications. Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining.
| Unit Size | 5 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml. |
| Solution | 10mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 5.0 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Format | Concentrate |
For even higher sensitivity and specific applications, see Fluorescein Avidin DCS.
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