Avidin D, Texas Red® labeled, is a highly fluorescent conjugate of Avidin D and sulforhodamine 101. Since the excitation and emission maxima are well separated from those of fluorescein, Texas Red® Avidin D can be employed with Fluorescein Avidin D or other fluorescein conjugates to simultaneously localize two antigens in the same tissue section.
|Unit Size||5 mg|
|Applications||Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation|
|Recommended Usage||For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended concentration range for use is 5-30 µg/ml.|
|Solution||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide|
|Maximum Excitation||595-604 nm|
|Maximum Emission||606-615 nm|
|Recommended Storage||2-8 °C|
|Concentration||2.5 mg active conjugate/mL|
|Color of Fluorescence||Red|
Texas Red® Avidin D is also ideal for flow cytometry using instruments equipped with dye lasers.
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