Chromogen Color Blue | |
Detection Enzyme Peroxidase | |
Microscopy Brightfield, Spectral Imaging | |
Mounting Medium Type Non-Aqueous (Permanent) | |
Unit Size 1 Kit | |
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Safety Title WARNING: Cancer –www.P65Warnings.ca.gov | |
Substrate Reaction Product Precipitating Reaction Product |
TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine)
TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.
The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.
Features:
• Greater sensitivity than conventional substrates
• Stock solutions supplied in convenient dropper bottles promoting ease of handling
• No wait times for mixing and dissolving powders or tablets
• Sufficient reagents to produce 300 ml of working solution
$199.00
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Kit Components
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Technical Information TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays. Counterstain / Substrate CompatibilityThis table is designed as a reference to determine the optimal counterstain / substrate combination for your application. Consideration should be given to tissue type, antigen unmasking protocol, and other detection parameters to achieve the desired staining intensity.
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