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Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) (SA-5014-1)

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Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size1 mg
ApplicationsBlotting Applications, Elispot, ELISAs
Recommended UsageDo not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Citations

Technical Information

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.

Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

This Horseradish peroxidase streptavidin preparation (HRP-Sav; SA-5014) is optimized for use in solid-phase assays such as ELISAs and blotting applications.

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Applicable patents and legal notices are available at legal notices.

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