The Cy7 azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
Cy7 Azide is a bright and photostable near-IR probe that is spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. The fluorescence of Cy7 Azide is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Abs/Em Maxima | 753/775 nm |
| Extinction Coefficient | 255,000 |
| Spectrally Similar Dyes | Alexa Fluor® 750, RDye® 750, CF® 750 Dye, DyLight® 750 |
| Molecular weight | 1083.32 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Dark green solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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