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Cy5 Alkyne (CCT-TA116)

[text-backorder]

This product was previously sold as 1326 from Fluoroprobes.

Description

The far-red fluorescent Cy5 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 633 nm or 647 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Cy5 Alkyne can be reacted with azides (N3) via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Cy5 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

This is sulfonated dye is also known as sulfo-Cyanine5.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima649/671 nm
Extinction Coefficient
250,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar DyesAlexa Fluor® 647, CF™ 647 Dye, DyLight™649
Molecular weight787.96 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy5

Selected References

  1. Banerjee, C., et al. (2022). Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET. J Vis Exp., 186. [PubMed]
  2. Schamber, M. R., et al. (2022). Conformational fingerprinting of allosteric modulators in metabotropic glutamate receptor 2. Elife, 11:e78982. [PubMed]
  3. Schamber, M. R., et al. (2022). Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning. Nat Commun.13 (1), 2194. [PubMed]
  4. Zhang, C., et al. (2016). In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy. Proc Natl Acad Sci U S A.113 (19), 5173-8. [PubMed]

Applicable patents and legal notices are available at legal notices.

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