Cy5 DBCO NHS Ester is a bright, far red fluorescent dual-label click chemistry probe that allows for simultaneous attachment of DBCO moiety and far red fluorescent Cy5 label. Conjugates prepared with Cy5 DBCO NHS Ester reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern Cy5 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Cy5 dye is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
Unit Size | 1 mg, 5 mg |
---|---|
Abs/Em Maxima | 649/671 nm |
Extinction Coefficient | 250,000 |
Flow Cytometry Laser Line | 633 or 635 nm |
Microscopy Laser Line | 633 or 635 nm |
Spectrally Similar Dyes | Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 |
Molecular weight | 1098.30 |
CAS | N/A |
Solubility | Water, DMSO, DMF |
Appearance | Blue solid |
Storage Conditions | -20°C. Desiccate |
Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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