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AZDye 647 DBCO (CCT-1302)

This product was previously sold as 1127 from Fluoroprobes.

AZDye™ 647 DBCO (formerly sold as AFDye™ 647 DBCO) is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Description

AZDye™ 647 DBCO is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AZDye™ 647 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AZDye™ 647 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 647 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima648/671 nm
Extinction Coefficient
270,000
Spectrally Similar DyesAlexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight1117.33
CASN/A
SolubilityWater, DMSO, DMF
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Af Af

Selected References

  1. Halliday, G. M., et al. (2022). Chemoselective Bioconjugation of Amyloidogenic Protein Antigens to PEGylated Microspheres Enables Detection of α-Synuclein Autoantibodies in Human Plasma. Bioconjug Chem., Online ahead of print. [PubMed]

Applicable patents and legal notices are available at legal notices.

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