AZDye™ 546 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable. In application where the presence of copper is a concern AZDye™ 546 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AZDye™ 546 dye is spectrally almost identical Alexa Fluor® 546, CF 543 and TAMRA.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||554/570 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
543 nm or 546 nm
|Spectrally Similar Dyes||Alexa Fluor® 546, Atto™ 546, CF® 546|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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