AZDye™ 488 DBCO is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AZDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, CF® 488 Dye and Alexa Fluor® 488.
DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademarks of Biotium Inc.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||494/517 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
|Spectrally Similar Dyes||FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488|
|Molecular weight||792.12 (protonated)|
|Solubility||Water, DMSO, DMF|
|Appearance||Orange to light red solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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