AZDye 488 DBCO

AZDye™ 488 DBCO is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AZDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor^® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight^® 488, CF^® 488 Dye and Alexa Fluor^® 488.

DyLight^® and Alexa Fluor^® are registered trademarks of Thermo Fisher Scientific. CF^® Dye is a registered trademarks of Biotium Inc.

Price range: $208.00 through $1,961.00

Category: AZ Dyes

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SKU: CCT-1278

CAS Number N/A
Molecular Weight 792.12 (protonated)
A260 Correction Factor 0.35
A280 Correction Factor 0.19
Appearance Orange to light red solid
Extinction Coefficient 73,000
Purity >95% (HPLC)
Unit Size 1 mg, 5 mg, 25 mg
Solubility Water, DMSO, DMF
Storage Instructions -20°C. Desiccate
Spectrally Similar Dyes FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Laser Line 488 nm
Excitation/Emission Maximum 494/517 nm
Shipping Conditions Ambient temperature
Shipping Instructions Ambient temperature

Abs/Em Spectra

Af488

Selected References

Loebel , C., et al. (2022). Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology. Nat Protoc., 10.1038, Online ahead of print. [PubMed]
Lancien, M., et al. (2020). A snake toxin as a theranostic agent for the type 2 vasopressin receptor. Theranostics., 10 (25), 11580-11594. [PubMed]
Simon P. Wisnovsky, et al. (2020). Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. PNAS, 117 (41), 25293-25301. [PNAS]
Loebel, C., et al. (2020). Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte–Hydrogel Interface. Adv. Funct. Mater. [PubMed]
Loebel, C., et al. (2019). Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels. Nature Materials, 18, 883-891. [PubMed]