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AZDye 594 Alkyne (CCT-1297)

[text-backorder]

This product was previously sold as 1106 from Fluoroprobes.

Description

AZDye™ 594 Alkyne is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.

For application where the presence of copper is not acceptable, please consider our AZDye™ 594 DBCO probe for copper-less detection of azide-modified molecules.

Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar DyesAlexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight758.18 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF594

Applicable patents and legal notices are available at legal notices.

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