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AZDye™ 568 NHS Ester (Alexa Fluor 568 NHS Ester equivalent) is an amine-reactive, orange fluorescent dye routinely used to label proteins or antibodies through the primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. AZDye™ 568 dye can be used for proteins labeling at high molar ratios without significantself-quenching, enabling brighter conjugates and more sensitive detection.
AZDye™ 568 is a water-soluble, photostable, and bright orange dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 568 dye ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. AF™ 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
---|---|
Reactivity | Primary amine |
Abs/Em Maxima | 578/602 nm |
Extinction coefficient | 88,000 cm-1M-1 |
Solubility | Water, DMSO, DMF |
Spectrally similar dyes | Alexa Fluor® 568, CF™ 568 |
Molecular weight | 791.80 (protonated) |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
[protein-labeling-calculator]
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