AZDye™ 568 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable. In application where the presence of copper is a concern AZDye™ 568 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 568 dye structurally is identical to Alexa Fluor® 568. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including CF® 568 Dye, Alexa Fluor® 568, Rhodamine Red and ATTO 565
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 578/602 nm |
| Extinction Coefficient | 88,000 |
| Flow Cytometry Laser Line | 532 nm or 568 nm |
| Microscopy Laser Line | 532 nm or 568 nm |
| Spectrally Similar Dyes | Alexa Fluor® 568, CF® 568 |
| Molecular weight | 953.04 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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