AZDye™ 546 Azide is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 546 is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||554/570 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
543 nm or 546 nm
|Spectrally Similar Dyes||Alexa Fluor® 546, Atto™ 546, CF® 546|
|Molecular weight||944.10 (protonated)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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