AZDye™ 568 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
AZDye™ 568 is a bright, and highly photostable, red-fluorescent probe optimally excited by the 532 nm or 568 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||578/602 nm|
|Spectrally Similar Dyes||Alexa Fluor® 568, CF™ 568|
|Solubility||Water, DMSO, DMF, MeOH|
|Appearance||Dark blue solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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