AZDye™ 555 Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AZDye™ 555 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AZDye™ 555 Azide can be used a less expensive alternative to Alexa Fluor® 555 Azide.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 555/572 nm |
| Extinction Coefficient | 155,000 |
| Flow Cytometry Laser Line | 532 nm or 555 nm |
| Microscopy Laser Line | 532 nm or 555 nm |
| Spectrally Similar Dyes | Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye |
| Molecular weight | 915.08 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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