This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
| Unit Size | 5 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology |
| Recommended Usage | Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. DO NOT VORTEX! |
| Recommended Storage | 2-8 °C |
| Conjugate | Unconjugated |
| Sugar Specificity | N-Acetylgalactosamine |
Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
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