Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.
Biotinylated Ulex europaeus agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
|Unit Size||2 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology|
|Recommended Usage||For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.|
|Recommended Storage||2-8 °C; Store frozen for long term storage|
|Solution||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide.|
|Concentration||2 mg active conjugate/ml|
|Sugar Specificity||Fucose, Arabinose|
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose
To request a quote for products: