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Streptavidin, Texas Red™ (SA-5006-1)

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Description

Texas Red Streptavidin is a highly fluorescent conjugate of streptavidin and sulforhodamine 101. It can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possesses very low non-specific binding properties
  • Has a high fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum: 595 nm
  • Emission maximum: 615 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageThe recommended concentration range for use is 5-30 µg/ml.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation595-604 nm
Maximum Emission606-615 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateTexas Red
Color of FluorescenceRed
FormatConcentrate

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Since the excitation and emission maxima are well separated from those of fluorescein, Texas Red Streptavidin can be employed with Fluorescein Streptavidin or other fluorescein conjugates to simultaneously localize two antigens in the same tissue section. Texas Red Streptavidin is also ideal for flow cytometry using instruments equipped with dye lasers.

Applicable patents and legal notices are available at legal notices.

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