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Texas Red™ Avidin DCS, Cell Sorting Grade (A-2016-1)

The product has been discontinued

Please consider the following as potential substitutes: A-2011-1. Fluorescein Avidin DCS, Cell Sorting Grade. Or contact Technical Support @ [email protected] for assistance in finding alternative.

Description

Avidin DCS, Texas Red® labeled, is a cell sorter grade of Texas Red® Avidin D. Through the development of a new method of labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Texas Red® Avidin DCS have a high F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentration for staining of cells or tissues is 5-20 µg/ml.
Solution10 mM HEPES, 0.5 M NaCl, pH 7.5, 0.08% sodium azide, 10 mg/ml beta cyclodextrin
Maximum Excitation595-604 nm
Maximum Emission606-615 nm
Recommended Storage2-8 °C
Concentration2.0 mg active conjugate/ml
ConjugateTexas Red
Color of FluorescenceRed
FormatConcentrate

Citations

Technical Information

Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D.
 
  • Excitation maximum:  595 nm
  • Emission maximum:  615 nm
  • Color:  Red

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