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Fluorescein Avidin DCS, Cell Sorting Grade (A-2011-1)

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Description

Avidin DCS, Fluorescein labeled, is a cell sorter grade of Fluorescein Avidin D. Through the development of a new method of fluorescein labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Fluorescein Avidin DCS have an optimal F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor staining cells or tissues using fluorescent microscopy, the recommended concentration range is 5-30 µg/ml with incubation times of 15-30 minutes.
Solution10 mM HEPES, 0.5 M NaCl, pH 8.0, 0.08% sodium azide, 10 mg/ml beta-cyclodextrin
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Recommended Storage2-8 °C
Concentration2.0 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen

Citations

Technical Information

Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D. 

  • Excitation maximum:  495 nm
  • Emission maximum:  515 nm
  • Color:  Green

Applicable patents and legal notices are available at legal notices.

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