Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight^™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight^™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight^™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.