DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
|Unit Size||1 mg|
|Applications||Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation|
|Recommended Usage||Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.|
|Solution||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.|
|Maximum Excitation||493 nm|
|Maximum Emission||518 nm|
|Recommended Storage||2-8 °C|
|Concentration||1.0 mg/ml active conjugate|
|Color of Fluorescence||Green|
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin.
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
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