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Streptavidin, Alkaline Phosphatase (SA-5100-1)

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Description

Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
  • Specific covalent linkages are chosen to provide stable, highly active conjugates

Specifications

Unit Size1 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageThe recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage2-8 °C
Detection Enzyme(s)Alkaline Phosphatase
ConjugateAlkaline Phosphatase
FormatConcentrate

Citations

Applicable patents and legal notices are available at legal notices.

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