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Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. (BP-1100-50)

The product has been discontinued

Please consider the following as potential substitutes: BA-1100-1.5. Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated. Or contact Technical Support @ [email protected] for assistance in finding alternative.


The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots. 


  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 


Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target SpeciesRabbit
Host SpeciesHorse


Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

These antibody solutions are provided in convenient dropper bottle format for ease of use.

Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications.

This antibody can be detected with the VECTASTAIN® ABC systems or other (strept)avidin reagent

Applicable patents and legal notices are available at legal notices.