Vector Laboratories is closed to Labor Day (US Holiday) on Monday, September 2nd.
We will be back in the office on Tuesday, September 3rd. We will respond to emails upon our return. Have a wonderful day.

Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated (BA-1100-1.5)

[text-backorder]

Description

The biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Supplied in solution 

Specifications

Unit Size1.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration1.5 mg/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide. Immunohistochemical grade bovine serum albumin has been added for stabilization.
Recommended UsageThe recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This is not the same antibody as that which is provided in the VECTASTAIN® ABC kits.
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesHorse
FormatConcentrate

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

With some exceptions the recommended dilution for most applications is 1:200.

Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications. This antibody can be detected with the VECTASTAIN ABC systems or other (strept)avidin reagents.

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Sign in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote