The biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Unit Size | 1.5 mg |
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Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
Concentration | 1.5 mg/ml |
Recommended Storage | 2-8 °C; Store frozen for long term storage |
Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide. Immunohistochemical grade bovine serum albumin has been added for stabilization. |
Recommended Usage | The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note:This is not the same antibody as that which is provided in the VECTASTAIN® ABC kits. |
Target Species | Rabbit |
Conjugate | Biotinylated |
Host Species | Horse |
Format | Concentrate |
With some exceptions the recommended dilution for most applications is 1:200.
Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications. This antibody can be detected with the VECTASTAIN ABC systems or other (strept)avidin reagents.
Applicable patents and legal notices are available at legal notices.
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