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PB NHS Ester (FP-1245)

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Description

PB dye (Pacific Blue™) is a bright, blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. The excitation and emission spectrum of PB dye offers minimal overlap with green fluorophore emission spectra, making it an ideal choice for the first violet channel. Conjugates of this dye are strongly fluorescent even at neutral pH.

The amine-reactive PB NHS Etser (Pacific Blue™ succinimidyl ester) can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ~410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amines
Abs/Em Maxima416/451 nm
Extinction coefficient30,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 405, DyLight® 405, Cascade Blue
Molecular weight339.21
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

pacific blue

Protein Labeling Calculator

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