MPS (NHS-3-maleimidopropionate) (QBD-10217)

Description

MPS (NHS-3-maleimidopropionate), product number QBD-10217, is the N-hydroxysuccinimidyl (NHS) ester of 3-(maleimido)propanoic acid and is used to crosslink sulfhydryls with amines. This product does not contain a dPEG® spacer between the reactive groups. The distance is six atoms (6.0 Å) from the reactive site of the maleimide to the carbonyl carbon of the propanoic acid.

The maleimido group reacts with sulfhydryl groups to form thiol ether bonds. In the range of pH 6.5 – 7.5, the thiol-maleimide reaction is chemoselective. Above pH 7.5, the maleimide group can also react with free amine groups. Consequently, high pH buffers should be avoided when using this product.

NHS esters are widely popular for reactions of carboxylic acids with primary or secondary amines to form stable amide bonds. Although the NHS ester hydrolyzes rather easily in water or aqueous buffer, this product can be dissolved in a water-miscible solvent such as N,N’-dimethylacetamide (DMAC) that has been dried over 3 Å molecular sieves. The solution of MPS is then added to the water or aqueous buffer to start the reaction.

Specifications

Documents

References

1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
2. Reagents for Astatination of Biomolecules. 2. Conjugation of Anionic Boron Cage Pendant Groups to a Protein Provides a Method for Direct Labeling that is Stable to in Vivo Deastatination. D. Scott Wilbur, Ming-Kuan Chyan, Donald K. Hamlin, Robert L. Vessella, Timothy J. Wedge, and M. Frederick Hawthorne. Bioconjugate Chem. 2007, 18 (4) pp 1226–1240. June 21, 2007. DOI: 10.1021/bc060345s.
3. Biodistribution of 211At labeled HER-2 binding affibody molecules in mice. Ann-Charlotts teffen, Ylva Almqvist, Ming-Kuan Chyan, Hans Lundqvist, Vladimir Tolmachev, D. Scott Wilbur and Jorgen Carlsson. Oncology Reports. 2007, 17 (5) pp 1141-1147. May 1, 2007. DOI: www.spandidos-publications.com/or/17/5/1141.