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MagnaLINK® Streptavidin Magnetic Beads (2.8 µm) (M-1003)

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Description

MagnaLINK 2.8 µm Streptavidin Magnetic Beads offer the highest biotin binding capacity on the market, combined with exceptional size uniformity. Higher binding translates to reduced bead mass required to immobilize a biotinylated sample and lower background from nonspecific binding, resulting in better signals and lower costs. MagnaLINK Streptavidin Magnetic Beads are 2.8 micron diameter, super-paramagnetic, hydrophilic polymer–encapsulated (no exposed iron), monodispersed microspheres with a uniform size distribution and a fast (<2 min) magnetic response time. They are colloidally stable with or without non-ionic detergents. The key to high biotin binding is in the unique covalently crosslinked streptavidin, based on ChromaLINK® technology. The high surface area, when combined with our efficient linking chemistry, produces a consistent product with a free biotin binding capacity of at least 12 nmol per milligram of beads. MagnaLINK beads also demonstrate exceptional size uniformity of <5% CV, as evidenced by scanning electron microscopy (SEM). These combined technologies make MagnaLINK beads the ideal magnetic bead for high-throughput robotic applications.

Specifications

 
Bead Size2.8 µm
Loading Capacity≥ 10 nmol/mg
ReactivityBiotin
ApplicationsAntibody Labeling, Aptamers, Next-Generation Sequencing (NGS), Photocrosslinking Studies
Recommended Storage2° – 8°C – Do Not Freeze

Citations

Technical Information

 

Product Description

MagnaLINK Streptavidin Magnetic beads are highly uniform, 2.8 µmeter polymer-encapsulated (no exposed iron), super-paramagnetic beads containing covalently cross-linked streptavidin (Figure 1). MagnaLINK streptavidin magnetic beads are made by covalently cross-linking streptavidin to a hydrophilic surface using the SoluLINK conjugation technology. The high surface area of these paramagnetic beads when combined with the efficient SoluLINK linking technology produces the most consistent and highest biotin binding capacity (≥10 nmol/mg) of any uniform streptavidin magnetic bead on the market. Beads are supplied at 1% solids (10 mg/mL) in nuclease-free water with 0.05% sodium azide.

M 1003 F1 jpg M-1003_F1
Figure 1. Scanning electron microscope image of MagnaLINK Streptavidin Magnetic beads.
M 1003 F3 jpg M-1003_F3
Figure 2. Cross-sectional diagram of MagnaLINK Streptavidin Magnetic bead.
 

Features of our MagnaLINK beads

  • Highest free biotin binding capacity of any uniform bead (≥ 10 nmol/mg)
  • Binds 0.8 nmol/mg biotinylated oligonucleotide
  • Binds 0.75 nmol/mg biotinylated-IgG @ 4 biotins/IgG
  • Beads are encapsulated (no exposed iron)
  • Paramagnetic beads are highly uniform in size (2.8 ± 0.2 microns)
  • Fast magnetic response time (60% w/w magnetite)
 

Binding Capacity Comparison: MagnaLINK Streptavidin Magnetic Bead Vs. Competitor’s Bead of Similar Size

LigandMagnaLINK 2.8 µm Binding CapacityCompetitor’s 2.8 µm Bead Binding Capacity
Free biotin≥ 10 nmol/mg0.90 nmol/mg
Biotinylated Oligo (23-mer)≥ 0.8 nmol/mgN/A
Biotinylated IgG (4 biotins per IgG)750 pmol/mg (113 µg/mg)60 pmol/mg (10 µg/mg)

MagnaLINK References:

  1. Moore R, Worrallo M, Mitchell P, Harriman J, Glen K, Thomas R. Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactorBMC Biotechnology volume 17, Article number: 65 (2017).

Applicable patents and legal notices are available at legal notices.

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