m-dPEG®₄-acid (QBD-10234)

Description

m-dPEG®4-acid, product number QBD-10234, is a short, methyl-capped, discrete chain length polyethylene glycol (dPEG®) spacer. The reactive end of the molecule terminates in a propionic acid group. The terminal propionic acid moiety can be coupled directly to free amines using EDC or another carbodiimide. Alternatively, the reactive end can be functionalized with N-hydroxysuccinimide (NHS); 2,3,5,6-tetrafluorophenol (TFP); 2,3,4,5,6-pentafluorophenol (PFP); or some other activating agent for reaction with amines.

m-dPEG®4-acid can modify amine-functionalized surfaces (carbon nanotubes, other nanoparticles, quantum dots, etc.) or free amines on biomolecules. When used to coat surfaces or modify biomolecules, m-dPEG®4-acid reduces, and often eliminates, non-specific binding and increases hydrophilicity. Please note that modification of surface amines on biomolecules with this uncharged, methyl-capped dPEG® spacer may alter the overall charge of the resulting conjugates.

In published scientific reports, m-dPEG®4-acid has been used in the following applications:
surface modification of polyelectrolyte multilayers;
thin films used for the detection of viable pathogenic microorganisms; and,
peptide modification.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Tunable Resistive m-dPEG Acid Patterns on Polyelectrolyte Multilayers at Physiological Conditions: Template for Directed Deposition of Biomacromolecules, Srivatsan Kidambi, Christina Chan, and Ilsoon Lee. Langmuir. 2008, 24 (1), pp 224–230. December 1, 2007. DOI: 10.1021/la702925r.

Selective Depositions on Polyelectrolyte Multilayers: Self-Assembled Monolayers of m-dPEG Acid as Molecular Template, Srivatsan Kidambi, Christina Chan, and Ilsoon Lee. J. Am. Chem. Soc. 2004, 126 (14), pp 4697–4703. March 17, 2004. DOI: 10.1021/ja039359o.

Functional PEG-Modified Thin Films for Biological Detection, Aaron S. Anderson, Andrew M. Dattelbaum, Gabriel A. Montano, Dominique N. Price, Jurgen G. Schmidt, Jennifer S. Martinez, W. Kevin Grace, Karen M. Grace, and Basil I. Swanson. Langmuir. 2008, 24 (5), pp 2240–2247. January 30, 2008. DOI: 10.1021/la7033438.

Arrays of lipid bilayers and liposomes on patterned polyelectrolyte template. Neeraj Kohli, Sachin Vaidya, Robert Y. Ofoli, Robert M. Worden, Illsoon Lee. Journal of Colloid and Interface Science. 2006, 301 (2) pp 461-469. January 2006. DOI:10.1016/j.jcis.2006.05.048.

DPP-IV-resistant, long-acting oxyntomodulin derivatives. Alessia Santoprete, Elena Capito, Paul E. Carrington, Alessandro Pocai, Marco Finotto, Annunziata Langella, Paolo Ingallinella, Karolina Zytko, Simone Bufali, Simona Cianetti, Maria Veneziano, Fabio Bonelli, Lan Zhu, Edith Monteagudo, Donald J. Marsh, Ranabir SinhaRoy, Elisabetta Bianchia,and Antonello Pessi. Journal of Peptide Science. 2011, 4 (17), pp 270-280. February 3, 2011. DOI 10.1002/psc.1328.

Polyelectrolyte Multilayer Stamping in Aqueous Phase and Non-Contact Mode. Sumit Mehrotra, Ilsoon Lee, Chun Liu, and Christina Chan. Industrial & Engineering Chemistry Research. 2011, 50 (15) pp 8851 – 8858. January 21, 2011. DOI:10.1021/ie102011m.

Determination of bacterial viability by selective capture using surface-bound siderophores. Mark L. Wolfenden, Rama M. Sakamuri, Aaron S. Anderson, Lakshman Prasad, Jurgen G. Schmidt, Harshini Mukundan. Advances in Biological Chemistry. 2012, (2) pp 396-402. September 30, 2012. DOI: 10.4236/abc.2012.24049.

New optical probes for the continuous monitoring of renal function. Richard B. Dorshow, Bethel Asmelash, Lori K. Chinen, Martin P. Debreczeny, Richard M. Fitch, John N. Freskos, Karen P. Galen, Kimberly R. Gaston, Timothy A. Marzan, Amruta R. Poreddy, Raghavan Rajagopalan, Jeng-Jong Shieh, William L. Neumann. Proc. SPIE 6867, Molecular Probes for Biomedical Applications II. 2008. 68670C. February 13,2008. doi:10.1117/12.763697.

New optical probes for the continuous monitoring of renal function. Richard B. Dorshow, Bethel Asmelash, Lori K. Chinen, Martin P. Debreczeny, Richard M. Fitch, John N. Freskos, Karen P. Galen, Kimberly R. Gaston, Timothy A. Marzan, Amruta R. Poreddy, Raghavan Rajagopalan, Jeng-Jong Shieh, William L. Neumann. Proc. SPIE 6867, Molecular Probes for Biomedical Applications II. 2008. 68670C. February 13,2008. doi:10.1117/12.763697.

Robust Sensing Films for Pathogen Detection and Medical Diagnostics. Aaron S. Anderson ; Andrew M. Dattelbaum ; Harshini Mukundan ;Dominique N. Price ; W. Kevin Grace ; Basil I. Swanson. Frontiers in Pathogen Detection: From Nanosensors to Systems., 2009, Proc. Of SPIE 7167. 71670q-1 February 18, 2009. 10.1117/12.809383.