GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.
DyLight™ 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight™ 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.
| Unit Size | 0.5 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. |
| Recommended Storage | 2-8 °C |
| Maximum Excitation | 592 nm |
| Inhibiting and/or Eluting Sugar | Inhibiting Sugar: 500 mM galactose (S-9003) or 100 mM raffinose. |
| Maximum Emission | 617 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2. |
| Concentration | 1 mg active conjugate/ml |
| Conjugate | DyLight 594 |
| Color of Fluorescence | Red |
| Sugar Specificity | Galactose |
Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.
GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
Applicable patents and legal notices are available at legal notices.
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