Carboxyrhodamine 110 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Carboxyrhodamine110 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Carboxyrhodamine110, a non-sulfonated version of Alexa Fluor® 488, is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. Its absorption/emission spectra is a similar to those of DyLight® 488, Alexa Fluor® 488.
Carboxyrhodamine110 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 501/523 nm |
| Extinction Coefficient | 74,000 |
| Flow Cytometry Laser Line | 488 nm |
| Microscopy Laser Line | 488 nm |
| Spectrally Similar Dyes | Fluorescein, Alexa Fluor® 488, DyLight® 488 |
| Molecular weight | 880.96 |
| CAS | N/A |
| Solubility | DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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